Spectroscopy & Imaging Facility Extensions
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We have spectroscopy instrumentation in our lab that we share with
the Cornell community for the measurement of absorption, fluorescence,
steady-state fluorescence anisotropy, and cuvette-based time-correlated single
photon counting (TCSPC) fluorescence measurements. Cary 300 absorption UV-Vis spectrophotometer - measures absorption spectra from 190-900 nm. It also has custom holders for measuring filter and dichroic transmission spectra. PTI QuantMaster QM-4 fluorimeter - measures fluorescence emission and excitation spectra, steady-state fluorescence time courses and polarization anisotropy. (Link to the PTI Felix GX Software Manual) BRC Imaging facility extended imaging modalities Fluorescence Lifetime and FLIM: We can provide time-correlated single photon counting (TCSPC) instrumentation for measuring fluorescence lifetimes, either for cuvette measurements or combined with the BRC imaging facility's Zeiss confocal/NLO systems for Fluorescene Lifetime Imaging Microscopy (FLIM). TCSPC is carried out using a Becker & Hickl SPC-830 card and pulsed exciation sources are either a tunable femtosecond NIR pulsed laser (2P excitation) or a 445 nm pulsed diode laser. Time-resolved anisotropy measurements are also possible using the TCSPC instrumentation.
Fluorescence Correlation Spectroscopy (FCS) and Two-color Fluorescence Cross-Correlation Spectroscopy (FCCS):
We can provide expertise to help researchers needing the FCS capabilities of the BRC Imaging Facility's
Zeiss LSM systems for measuring molecular diffusion and interactions in solution or in cells.
FCS/FCCS is carried out using Zeiss' FCS modules in either confocal or two-photon mode.For access or consultation contact Dr. Warren Zipfel (wrz2@cornell.edu) |